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DECADES OF RESEARCH

To view more about each finding, click on any of the timeline discoveries below:

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1997

KLOTHO GENE DISCOVERED BY JAPANESE GROUP KUROSU ET AL (READ MORE)

The aging-suppressor gene that was identified by Kurosu et al in 1997 was named Klotho. The overexpression of the mouse α-Klotho gene slowed the aging process and extended the life span by 20–30%.

 
 
 

2005

KUROSU ET AL DEMONSTRATED THAT THE OVEREXPRESSION OF KLOTHO PROTEIN INHIBITED INSULIN (INSULIN GROWTH FACTOR 1) SIGNALING AND INCREASED LIFESPAN (READ MORE)

Suppression of aging in mice by the hormone Klotho: Klotho protein functions as a circulating hormone that binds to a cell-surface receptor and represses intracellular signals of insulin and insulin-like growth factor 1 (IGF1), an evolutionarily conserved mechanism for extending life span. Inhibiting insulin IGF1 signaling activates transcription factors that increase cellular protection against oxidative stress and may contribute to suppression of aging.

 
 
 

2006

REGULATION OF FIBROBLAST GROWTH FACTOR-23 SIGNALING BY KLOTHO: The Klotho protein directly binds to multiple FGF receptors (FGFRs).

 
 
 

2008

THE PHYSIOLOGICAL RELEVANCE OF AMPK IS DEMONSTRATED AND ITS ROLE IN THE REGULATION OF ENERGY HOMEOSTASIS IS ESTABLISHED (READ MORE)

AMPK: a metabolic gauge regulating whole body energy homeostasis: Science accumulates evidence that AMPK is involved in the regulation of energy balance at the whole-body level by responding to hormones and nutrient signals, which leads to changes in energy homeostasis.

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2014

SNOW ALGAE DISCOVERED TO STIMULATE ACTIVITY OF AMK PROTEIN (READ MORE)

In cell culture assays, the Snow Algae extract was found to induce a calorie restriction-mimetic effect by stimulating the expression of the Klotho gene and the activity of the AMPK protein. The extract reduced the loss in collagen expression in aged cells.

In lab tests, aged fibroblast cells exhibiting only 48% expression of the Klotho gene showed an upregulation to 147% upon being exposed to Snow Algae.

 
 
 

2018

KLOTHO SKIN IS THE FIRST TO ISOLATE HUMAN FIBROBLASTS CELL & CULTURE THEM IN SNOW ALGAE TO GET THE HIGHEST LEVEL OF EGF (READ MORE)

Then isolate, purify, and formulate them as the Second Generation Growth Factor Cosmeceuticals. The biotechnology firm publishes two studies in support of its techniques & formulations.

 
 
 

 
 
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REVOLUTIONARY STUDIES

STUDIES PERFORMED BY GAIL HUMBLE M.D. & THE KLOTHO SKIN TEAM

Additional studies are underway and will be published soon.

 
 
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SNOW ALGAE EFFECT ON FIBROBLAST CELLS AND MESENCHYMAL STEM CELLS GROWTH (READ MORE)

Study/Phase #1: Observation of general growth profile of cells in presence of 2% (w/v) snow algae.

 
 

 
 
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PROLIFERATIVE & PLEIOTROPIC EFFECTS (READ MORE)

CULTURING HUMAN FIBROBLAST CONDITIONED MEDIA - Since the discovery of the Klotho gene in 1997, scientists and medical doctors have been exploring the potential of regulating the Klotho gene to combat premature signs of aging. Sourced from the Swiss Alps, snow algae species, Chlamydomonas Nivalis has been recognized as an anti-aging active ingredient employed to protect and activate two key factors of the calorie restriction pathway: the Klotho longevity gene and the AMPK energy sensor.

Klotho Skin is the first biotechnology company to integrate Snow Algae in culturing fibroblast conditioned media to reveal the proliferative and pleiotropic effects—increased production of growth factors, cytokines and antioxidants to effectively suppress premature signs of aging.

DISCOVERY OF THE AGING GENE (READ MORE)

THE MOST EFFECTIVE ANTI-AGING COSMECEUTICAL: KLOTHO SKIN - Discovered in 1997 by Japanese group Kurosu et al., the Klotho gene is expressed in many tissues and cell types. Further research has validated over-expression of the Klotho gene in mammals extends lifespan due to it’s ability to mimic the caloric restriction pathway of cells. The caloric restriction pathway is one of the evolutionary conserved mechanisms for suppressing aging due to the moderate inhibition of insulin, which increases antioxidants and DNA repair while decreasing free radical damage and oxidative stress in cells.

Scientists and medical doctors have been exploring the potential of regulating the Klotho gene to combat premature signs of aging. Sourced from the Swiss Alps, snow algae species, Chlamydomonas Nivalis has been recognized as an anti-aging active ingredient employed by Klotho Skin to protect and activate two key factors of the calorie restriction pathway: the Klotho longevity gene and the AMPK energy sensor. Klotho Skin is a biotechnology company producing pharmaceutical-grade cosmetics that integrate Snow Algae in fibroblast cell conditioned media to produce next generation growth factor cosmeceuticals with elevated levels of EGF to effectively suppress aging.

 
 

 
 
 
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KLOTHO SKIN FORMULATION

Discovery Process

For over 20 years, the founder and team at Klotho Skin have been studying the regenerative properties of the Klotho gene for implementation within skincare. Klotho Skin is dedicated to our decades-long and continued research alongside the responsibility to disclose all of our innovative findings.

 
 

RESEARCH GOALS


  • To create a cosmetic serum that will provide anti aging benefits using a unique system of growth factors and biomimetic peptides

  • To introduce a serum that goes beyond what has been available and uses second generation growth factors to work on a genetic level

  • To introduce a serum that is an anti inflammatory as well as an antioxidant

 

TRUTHS


1. GROWTH FACTORS (READ MORE)

Fibroblast, otherwise known as skin cells, are one of the first cells to respond to wound healing by secreting communication signals termed growth factors (GFs) to signal cellular proliferation and repair.

As we age, the body produces less fibroblast cells and less growth factors which inhibits the body’s ability to defend itself against UV radiation and harmful environmental factors.

Exposure to extreme environments of high temperatures, stress, poor nutrition, lack of sleep and causes free-radical damage and increased oxidative stress in cells.


2. KLOTHO GENE (READ MORE)

Increased expression of Klotho results in an increase expression of FOXO1; which ultimately results in an increased resistance to oxidative stress as well as longevity and anti-aging.

The Klotho gene has the ability to inhibit IGF-1 signaling, an evolutionarily conserved mechanism for extending life span.

Klotho expression declines by 60% in aging skin cells.


3. SNOW ALGAE (READ MORE)

Studies showed 147% expression of Klotho when Snow Algae Powder was added to an aged fibroblast having only 47% expression of Klotho.

Snow Algae Powder mimics the effects of caloric restriction. Caloric restriction upregulates the Klotho gene, causing an up-regulation in AMPK (a cellular energy regulator) and activation of FOXO, which increases DNA repair and detoxification.

Implementing Snow Algae Powder into cell culturing process can further reduce the effects of aging in regards to adhesion, antioxidant activity, DNA repair, electron transport, metabolism, protein synthesis, collagen production, and stress response.

 

TECHNIQUES


1. PREPARATION OF FORMULATIONS OF PRIMARY MEDIA (READ MORE)

Klotho Skin developed a proprietary primary fibroblast basal media (base cell structure) consisting of defined concentrations of Snow Algae to test cell proliferation. The media bathed in 1% Snow Algae showed the highest levels of growth factor proliferation.


2. CELL PROLIFERATION ASSAY (READ MORE)

Human fibroblast cells were plated and allowed to seed in primary fibroblast media that did not contain Snow Algae. After seeding, they were bathed in Snow Algae twice. The resultant high concentrations of high performing growth factors were then harvested.


3. GROWTH FACTOR, INFLAMMATORY CYTOKINES MEASUREMENTS (READ MORE)

Media harvested from proliferating fibroblasts exposed to Snow Algae-conditioned media (at 1%) was tested for multiple growth factors as well as multiple inflammatory cytokines.

*No biologically live elements are utilized in Klotho Skin ingredients.

 

RESULTS


Klotho Skin is the first skin care line to patent the process of taking human fibroblasts cells and culturing them in Snow Algae Powder to upregulate the klotho gene in the fibroblast. This results in the production of high concentrations of Epidermal Growth Factors.

Epidermal growth factor EGF is an age-preventative  ingredient that supports cell renewal and wound repair.It has potent regenerative properties making it critical for age prevention and age reversal of the skin. It is one of the many growth factors that are isolated, purified and collected.

These second generation growth factors are combined with additional snow algae and other complementary ingredients to create a unique formulation that turns back the clock on your own fibroblasts.

As adult fibroblast cells age, collagen production drops almost in half.  Studies have shown that  fibroblasts treated with our process can begin producing collagen like a younger fibroblast cell. In addition to maximizing the cells’ Klotho gene expression, we are able to initiate an anti-inflammatory process and the reversal of oxidative stress to skin cells, creating the appearance of younger and healthier skin.

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DISCOVER KLOTHO SKIN


 
 
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